For a high speed USB 2.0 PCB, I've read that it should have a continuous, isolated ground plane. Since power is provided by USB that's pretty easy to ensure, just connect the ground plane to the ground pin on the USB connector. However, I'm using an LDO to drop the voltage from 5V to 3.3V (to power an SD card and UC3A3). I'm uncertain if the 3.3V ground plane should be connected (at one point) to the High Speed USB ground plane. Here's my reasoning:
Connect ground planes: If the ground planes are unconnected, the 3.3V ground is floating without a defined value. I'm concerned this could cause problems when D+ & D- and VBUS are connected to the UC3A3. Also, I'm not sure how the LDO would react to having a floating ground, I assume it would be ok as it's just going to separate Vout and GND by 3.3V (provided the input is an appropriate voltage/current).
Unconnected ground planes: This is technically what an "isolated" ground plane would be.